中国实验动物学报2011，Vol.19Issue(5)：361-365,5.DOI:10.3969/j.issn.1005-4847.2011.05.001
人骨髓间充质干细胞脑内移植对食蟹猴脑出血的治疗作用
Intracerebral transplantation of human bone marrow mesenchymal stem cells promotes the functional recovery in Macaca fascicularis with intracerebral hemorrhage
摘要
Abstract
Objective To investigate the therapeutic effect of human bone marrow-derived mesenchymal stem cell( hBMSC) transplantation on experimentally induced intracerebral hemorrhage in Macaca fascicularis, and to examine the effects of hBMSC transplantation on the recovery of neurological function. Methods In 12 Macaca fascicularis, autologous femoral arterial blood was injected into the brain parenchyma to develop an intracerebral hemorrhage (ICH) model. The animals were randomly divided into high-dose, low-dose, and control groups. After one week, 250 u,l of hBMSC in amount of 5 × 106, 1 × 106cells, or saline were injected at the edge of the hematoma, respectively. High field MR scanning and positron-emission tomography ( PET) scans were performed weekly before and after the hBMSC transplantation. Neurological functions were evaluated weekly, and the brains were taken for pathological examination at 4 weeks after hBMSC transplantation. Results After hBMSC transplantation, neural function scores were improved significantly in the high- and low-dose groups compared with that of the control group. Two weeks after transplantation, the surrounding area of the hematoma exhibited a circular elevated standardized uptake value (SUV) , and the SUV% of the putamen in the high-dose group was significantly higher than that of the control group ( P = 0. 02). At 3 weeks after transplantation, the SUV percentages of the putamen and thalamus in the high-dose group were significantly higher than that of the the Control group (P = 0. 03 and 0. 04, respectively). At 4 weeks after transplantation, pathological examination showed a large amount of neovasculature around the ICH after hBMSC transplantation in the transplantation groups compared with that in the control group (P <0. 01). Conclusions Intracerebral transplantation of human bone marrow mesenchymal stem cells can improve the functional recovery of damaged cerebral tissue in Macaca fascicularis. It provides important experimental evidence indicating the use of hBMSC to restore neurological deficits in clinical treatment of intracerebral hemorrhage.关键词
间充质干细胞/脑出血/移植/食蟹猴Key words
Bone marrow mesenchymal stem cells/ Intracerebral hemorrhage/ Transplantation/ Macaca fascicularis分类
生物科学引用本文复制引用
朱华,安沂华,赵春华,王任直,秦川,冯铭,卢珊,李秦,刘颖,陈云新,代小伟,李昊,孙井江..人骨髓间充质干细胞脑内移植对食蟹猴脑出血的治疗作用[J].中国实验动物学报,2011,19(5):361-365,5.基金项目
863课题(编号:2006AA02A115). （编号:2006AA02A115）
