安徽农业科学2011，Vol.39Issue(23)：13942-13943,13946,3.
4种高梁基因组DNA快速提取方法的比较
Comparative Study on Four Methods for Quick Extraction of Sorghum Genomic DNA
摘要
Abstract
[Objective] This study was to find out a quick, simple, and low-cost method for the extraction of sorghum genomic DNA. [ Method] Four plant genomic DNA extraction methods based on CTAB, including liquid nitrogen grinding method (method Ⅰ) , buffer grinding method (method Ⅱ) , drying grinding method (method Ⅲ) and directly grinding method (method Ⅳ), were used to extract the sorghum genomic DNA from leaves; further the quantity and quality of the yielded DNA were detected by gel electrophoresis, SSR-PCR and SRAP-PCR. [ Result ] These four methods performed no remarkable difference in DNA product. The method Ⅰ and method Ⅱ produced DNA with higher purity and better integrity, which, especially from method Ⅰ, is effective for SRAP-PCR and SSR-PCR. While the DNA extracted via method Ⅲ and method Ⅳ had less integrality and lower purity, and only effective in SSR-PCR. [Conclusion] Enough amount of sorghum genomic DNA to perform tens of PCR could be quickly extracted using all these four methods. The DNA obtained via method Ⅰ and method Ⅱ had a broader application spectrum (SRAP, RAPD, ISSR and SSR) than that via method Ⅲ and method Ⅳ which is only proper for PCR targeting small DNA fragments (SSR).关键词
高粱/叶片/基因组DNA/CTAB法/快速提取Key words
Sorghum bicolor/ Leaf/ Genomic DNA/ CTAB method/ Quick extraction分类
农业科技引用本文复制引用
高建明,夏卜成,杨洪,曲荣桂,桂枝,罗峰,裴忠有,孙守钧..4种高梁基因组DNA快速提取方法的比较[J].安徽农业科学,2011,39(23):13942-13943,13946,3.基金项目
国家科技支撑计划项目(2007BAD42B03). （2007BAD42B03）
