西安交通大学学报（医学版）2011，Vol.32Issue(5)：628-631,4.
丝素蛋白-羟基磷灰石类骨质复合生物材料的生物相容性
Biocompatibility of silk fibroin-hydroxyapatite osteoid composite biomaterials
摘要
Abstract
Objective To evaluate the biocompatibility of silk fibroin-hydroxylapatite osteoid composite biomaterials. Methods Osteoblast-like cell line MG-63 in the experiment group was cultured in vitro and vaccinated on the surface of silk fibroin-hydroxyapatite osteoid composite biomaterials, while in the control group it was inoculated on the pure hydroxyapatite. The biocompatibility of the materials in the two groups was evaluated by inverted microscopy and tetrazolium salt colorimetric (MTT) assay, respectively. For in vivo evaluation of biocompatibility, the silk fibroin-hydroxyapatite osteoid composite biomaterials were implanted in the femoral medullary cavity of New Zealand white rabbits in the experiment group, while pure hychoxyapatite were implanted in the control group. At week 4 and week 8, the specimens were removed, decalcified, embedded in paraffin, sectioned and stained (HE and Masson staining) to observe new bone formation and evaluate the biocompatibility in vivo. Results In vitro and in vivo experiments showed that silk fibroin-hydroxyapatite osteoid composite biomaterials in the experiment group had good biocompatibility. Conclusion Silk fibroin-hydroxyapatite has excellent biocompatibility, and thus it is expected to become a new type of bone substitute materials.关键词
丝素蛋白/羟基磷灰石/生物相容性Key words
silk fibroin/hydroxyapatite/biocompatibility分类
医药卫生引用本文复制引用
牛林,邹蕊,石福乔,逯宜,陈治清,刘启达..丝素蛋白-羟基磷灰石类骨质复合生物材料的生物相容性[J].西安交通大学学报（医学版）,2011,32(5):628-631,4.基金项目
陕西省科技攻关项目(No.200814K-03) （No.200814K-03）
