西安交通大学学报（医学版）2011，Vol.32Issue(5)：569-575,7.
Peroxiredoxin1基因沉默对肝癌细胞HepG2和SMMC-7721放射增敏的研究
Sensitizing hepatocellular carcinoma cells HepG2 and SMMC-7721 to ionizing radiation by peroxiredoxin 1 silencing
高麦仓 1张龙 2张殿增 3张军1
作者信息
- 1. 西安交通大学医学院第二附属医院消化科,陕西西安710004
- 2. 西安交通大学医学院第一附属医院肿瘤放疗科,陕西西安710061
- 3. 西安交通大学医学院实验中心,陕西西安710061
- 折叠
摘要
Abstract
Objective To investigate the role of small interference RNA (siRNA) of peroxiredoxin (Prx) 1 in the radiosensitizing human hepatocellular carcinoma cells. Methods Prx mRNA expression profiles in human HepG2 and SMMC-7721 cells were determined by reverse transcription polymerase chain reaction. Prx1 was silenced by small interference RNA (siRNA) in HepG2 and SMMC-7721 cells. The two cell lines were divided into three groups (blank control group, negative control group and Prxl siRNA transfected group). The effects of Prxl siRNA on proliferation of HepG2 and SMMC-7721 in the three groups were determined by colony-forming assay; the cell cycle, intracellular reactive oxygen species (ROS) and cell apoptosis were detected by flow cytometry. Results Prxl, Prx3 and Prx5 were highly expressed in HepG2 and SMMC-7721 cells. Prxl siRNA transfected group exhibited a decreased proliferation following ionizing radiation (IR), arrested G2-M checkpoint (12 hours after IR), more cells blocked in G2 phase, a higher apoptosis rate (24 hours after IR) and increased intracellular ROS levels (1 hour after IR) compared to those in the control groups (P<0.05). The sensitization enhancement ratio of Prxl siRNA transfected groups was between 1.38 and 1. 45. Conclusion Silencing Prxl sensitizes hepatocellular carcinoma cells to ionizing radiation in part through accumulation of intracellular reactive oxygen species and cell cycle arrest.关键词
肝癌细胞/放射敏感性/Peroxiredoxin 1/RNA干扰/细胞内活性氧/细胞周期阻滞/放射增敏Key words
hepatocellular carcinoma cell/radiosensitivity/peroxiredoxin 1/RNA interference/intracellular reactive oxygen species/cell cycle arrest/radiosensitizing分类
医药卫生引用本文复制引用
高麦仓,张龙,张殿增,张军..Peroxiredoxin1基因沉默对肝癌细胞HepG2和SMMC-7721放射增敏的研究[J].西安交通大学学报（医学版）,2011,32(5):569-575,7.
