西安交通大学学报（医学版）2011，Vol.32Issue(5)：585-588,4.
骨髓间充质干细胞对5/6肾切除大鼠肾脏损伤时Bcl-2、Bax表达的影响
Effects of bone mesenchymal stem cells on Bcl-2 and Bax expressions in the progression of renal injury in 5/6 nephrectomy rats
摘要
Abstract
Objective To study the protective effects of bone marrow mesenchymal stem cells (BMSCs) on 5/6 nephrectomy rats as well as observe the expressions of Bcl-2 and Bax proteins in the progression of renal injury. Methods BMSCs were isolated from male SD rats and labeled with PKH26 before injection. Totally 40 female recipient SD rats were divided randomly into four groups: sham group, DMEM-F12 infusion group (model group), benazepril gastric instillation group (control group) and BMSCs injection group (BMSCs group), each having 10 rats. After 60 days, the serum levels of BUN and SCr were observed. The expressions of Bcl-2 and Bax proteins were measured by immunohistochemical technique. The distribution of PKH26 positive BMSCs in the kidney of recipient SD rats was examined by immunohistochemistry. Results The renal function in sham group was significantly better than that in any other groups (P<0. 05); both control group and BMSCs group were significantly better than model group (P<0.05). Bcl-2 and Bax expressions and the ratio of Bcl-2/Bax were higher in model group than in the other groups (P<0.05); the expressions of Bcl-2 and Bax were higher in BMSCs group than in control group (P<0. 05). On the 60th day after 5/6 nephrectomy, BMSCs were found in renal tissues of BMSCs treated rats. Conclusion Exogenic BMSCs can decrease the expressions of Bcl-2 and Bax, lower the ratio of Bcl-2/Bax, reduce kidney cell apoptosis, and contribute to repairing the injured kidney.关键词
慢性肾损伤/骨髓间充质干细胞/细胞凋亡/Bcl-2/BaxKey words
chronic renal injury/bone mesenchymal stem cell/cell apoptosis/Bcl-2/Bax分类
医药卫生引用本文复制引用
袁浩峥,姚钢炼,付荣国,刘晓丹,张莉,赵丽燕,李向利,薛荣亮..骨髓间充质干细胞对5/6肾切除大鼠肾脏损伤时Bcl-2、Bax表达的影响[J].西安交通大学学报（医学版）,2011,32(5):585-588,4.基金项目
国家自然科学基金资助项目(No.81070590) （No.81070590）
西安交通大学台湾光华基金项目(No.GH0203212) （No.GH0203212）
