癌变·畸变·突变2011，Vol.23Issue(6)：406-409,415,5.DOI:10.3969/j.issn.1004-616x.2011.06.001
同源重组修复基因RAD51可提高PTEN缺失细胞基因组稳定性
Homologous recombination repair gene RAD51 could increase the genomic stability of PTEN deleted cells
摘要
Abstract
OBJECTIVE: To explore the effect of genomic stability of PTEN-deficient mice embryonic fibroblasts after transfection with RAD51, an important homologous recombination repair gene. METHODS: Immunofluorescence assay was used to observe the number of spontaneous y -H2AX foci. Neutral single cell gel electrophoresis was applied to detect spontaneous and radiation-induced DNA double-strand breaks in both PTEN-deficient and PTEN wild-type cells. RESULTS: PTEN deletion led to an increase of spontaneous y -H2AX foci and the length of tail moment, indicating DNA double-strand breaks. Transfeetion with RAD51 increased cell survival and decreased radiation-induced DNA double-strand breaks of PTEN-deficient cells. CONCLUSION: Homologous recombination gene RAD51 could increase genomic stability of PTEN-deficient cells.关键词
PTEN/RAD51/基因组稳定性Key words
PTEN/ RAD51/ genomic stability分类
生物科学引用本文复制引用
陈忠民,徐勤枝,胡迎春,霍艳英,周平坤,吴德昌..同源重组修复基因RAD51可提高PTEN缺失细胞基因组稳定性[J].癌变·畸变·突变,2011,23(6):406-409,415,5.基金项目
国家重大科学研究计划(2007CB914603),国家自然科学基金项目(30872136) （2007CB914603）
