癌变·畸变·突变2011，Vol.23Issue(6)：434-436,441,4.DOI:10.3969/j.issn.1004-616x.2011.06.007
60Co γ射线诱导正常人淋巴母细胞XPCmRNA表达的剂量相关性研究
Dose-dependent inducible expression of XPC mRNA in human normal lymphoblastoid cells by 60Co γ-rays
摘要
Abstract
OBJECTIVE: This study was designed to investigate the dose-dependency of XPC mRNA expression in normal human lymphoblastoid AHH-1 cells induced by 60Co γ-rays, and its possibility as a biomarker forradiation injury. METHODS: After irradiated with 0, 1, 2, 4, 6, 8 or 10 Gy of 60Co γ-rays, AHH-1 cells wereharvested at 4 h, 10 h and 24 h post-irradiation, followed by mRNA extraction, mRNA level was measured byquantitative real-time PCR. RESULTS : The mRNA expression level of XPC gene in the cells significantly increased asearly as 4 h after 0-10 Gy irradiation, and reached a first expression peak, lasting 10 h. Then, this radiation-inducedexpression continued increasing at least for 24 h and reached the second expression peak. The radiation-increasedexpression of XPC mRNA showed a dose-dependent manner within the dose range of 0-8 Gy. CONCLUSION: Theradiation-increased expression of XPC mRNA exhibited a significant dose- and time- dependency, with a potential fordeveloping into a novel radiation molecular biodosimeter.关键词
放射损伤/生物剂量计/XPC/基因表达/实时定量PCRKey words
radiation injury/ biodosimeter/ XPC/ gene expression/ real-time PCR分类
医药卫生引用本文复制引用
刘晓丹,张士猛,李兵,樊嵘,陈英,周平坤..60Co γ射线诱导正常人淋巴母细胞XPCmRNA表达的剂量相关性研究[J].癌变·畸变·突变,2011,23(6):434-436,441,4.基金项目
国家自然科学基金杰出青年基金(30825011) （30825011）
