江西农业大学学报2011，Vol.33Issue(5)：855-860,6.
青稞“昆仑12号”HVA1基因的克隆与蛋白质结构预测
Cloning of HVA 1 Gene and Predicting of Protein Structure in Hulless Barley Kunlun 12
摘要
Abstract
Drought resistance is one of the most important characters of stress tolerance ability in hulless barley. Based on the DNA sequence of HVAl gene in barley which is related to drought resistance, a pair of primers were designed. The specific fragments of about 700 bp in size were obtained, respectively, after PCR amplification using the seedlings Cdna of hulless barley variety Kunlun 12 under drought conditions after 8 h as template. Then they were cloned into vector Pmd -20T, and sequenced after the identification of the re-combinants and endonuclease analysis. The results indicated that compared with the strong drought resistance hulless barley in Tibetan, HVAl gene in Kunlun 12 shared 99. 5% and 99. 1% homology in nucleotide acid sequence and amino acid sequence, respectively. The gene included an open reading frame of 642 nuelcotides and encodes 213 amino acid residues rich in Ala, Thr, Lys. The protein coded by HVAl including 9 repeat-able conservative sequences belonged to group3 lea. Secondary structure analysis showed that the protein rich in super - hydrophilic alpha helix structure could provide a hydrophilic surface of hydrophobic areas when cells were dewatered and thus hulless barley variety Kunlun 12 had strong capability in drought resistance. The drought - resistant gene cloning laid the foundation for breeding Hulless Barley varieties with stress tolerance a-bility in Qinghai - Tibet plateau.关键词
青稞/HVA1基因/基因克隆/序列分析Key words
hulless barley/ HVA1 gene/ gene cloning/sequence analysis分类
农业科技引用本文复制引用
姚晓华,邓晓青,吴昆仑,张志斌..青稞“昆仑12号”HVA1基因的克隆与蛋白质结构预测[J].江西农业大学学报,2011,33(5):855-860,6.基金项目
农业部948(引进国际先进农业科学技术)项目(2010-Z29)和青海大学中青年科研基金项目(2010-QN-04) （引进国际先进农业科学技术）
