安徽农业科学2011,Vol.39Issue(28):17156-17157,2.
核桃外植体的组织培养
Tissue Culture of Walnut Explant
杨海波 1王娟 1周鹏程 1孟利峰 1高涛2
作者信息
- 1. 山西省农业科学院果树研究所,山西太谷030815
- 2. 山西农业大学园艺学院,山西太谷030801
- 折叠
摘要
Abstract
[Objective] The aim was to study the method of tissue culture and rapid propagation of walnut. [ Method] The explains were col lected from stem with axillary buds, the tube propagation of Jinboxiangl was studied. [ Result] The optimum culture medium for germination of the axillary buds: DKW + BA 1.5 mg/L; for differentiation and subculture:DKW + BA 0.4 mg/L + IBA 0.01 mg/L; for rooting;l/2 DKW + IBA 1.0 mg/L, however, rooting efficiency was only 23.3% , this result was not satisfied. The method of taking tool with two steps was ide al, and taking root efficiency reached to 36.7%. [ Conclusion ] The optimal culture conditions of rapid propagation of Jinboxiang 1 walnut were selected, which laid the foundation for constructing rapid propagation and amplifying walnut planting scale.关键词
核桃/外植体/组织培养Key words
Walnut/ Explanl, Tissue culture分类
农业科技引用本文复制引用
杨海波,王娟,周鹏程,孟利峰,高涛..核桃外植体的组织培养[J].安徽农业科学,2011,39(28):17156-17157,2.