安徽农业科学2011，Vol.39Issue(22)：13431-13433,3.
悬铃木方翅网蝽DNA提取及PCR检测
Extraction and PCR Detection of Total DNA of Corythucha ciliate Say
摘要
Abstract
[Objective] The research aimed to explore and establish a method of extraction of the total DNA from Corythucha ciliate Say. [ Method] The improved SDS method in combinaiton with a Silica adsorption column were used to extract the total DNA from Corythucha ciliate Say. The extracted DNA was qualitatively assessed for the density and purity through spectrophotometry and agarose gel electrophoresis. Further the mitochondrial gene Cytochrome Oxidase Subunit I(COI) and the 28S gene in Corythucha ciliate Say genome were amplified,respectively by PCR, followed by cloning and sequencing. [ Result] The results showed that the total DNA density of Corythucha ciliate Say was 52.8 μg/ml with a ratio of A260/A280 =2.06. In addition,the agarose gel electrophoresis band was clear. The bands of amplification outcome through PCR were clear and specific. The identify of the cloned fragment of the two gene preducts was further confirmed to be the Corythucha ciliate Say's COI and the 28S gene,respectively,after cloning and sequencing. [Conclusion] Using the improved SDS method high quality genomic DNA for further amplification usage could be obtain. The technique could conform with the needs of the future research of molecular biology.关键词
悬铃木方翅网蝽/DNA/提取/PCRKey words
Corythucha ciliate Say/DNA/Exlraction/PCR分类
农业科技引用本文复制引用
卢航,芶琳,李成磊..悬铃木方翅网蝽DNA提取及PCR检测[J].安徽农业科学,2011,39(22):13431-13433,3.基金项目
国家自然科学基金项目(30971795). （30971795）
