江西农业大学学报2011，Vol.33Issue(5)：987-992,6.
粘质沙雷氏菌中乙偶姻合成途径基因克隆、序列分析及表达
Cloning, Sequence Analysis and Heterogenous Expression of the Genes Involved in Acetoin Pathway from Serratia marcescens H30
摘要
Abstract
The aceA and aceB genes coding for α -acetolactate decarboxylase (α - ALDC) and α -ace-tolactate synthase (α - ALS) in the acetoin biosynthetic pathway from Serratia marcescens were identified and amplified through sequence alignment and Polymerase Chain Reaction (PCR). The sequencing results showed that the ORF lengths of aceA and aceB genes were 780 bp and 1 686 bp, and encoded proteins of 259 and 561 residues respectively. Furthermore, their molecular weights and isoelectric points of 28. % kD and 5.48, 69.70 kD and 5.88 were predicted, which were acidic proteins judging from the calculated pi values. Then both genes were ligated with the expression plasmid of pET28a( + ) and transformed into E. Coli BL21 (DE3) for their induced expression with IPTG. SDS - PAGE analysis revealed that there were two clear induced protein bands with molecular weights of about 29 kD and 60 kD on expected position. These results indicated that both genes of aceA and aceB were efficiently expressed in E. Coli BL21 (DE3 ).关键词
粘质沙雷氏菌/乙偶姻/克隆/序列分析/表达Key words
Serratia marcescens/ acetoin/ cloning/ sequence analysis/ heterogenous expression分类
生物科学引用本文复制引用
张燎原,孙建安,魏东芝,沈亚领..粘质沙雷氏菌中乙偶姻合成途径基因克隆、序列分析及表达[J].江西农业大学学报,2011,33(5):987-992,6.基金项目
国家“十一五”863专题(2006AA02Z243)和生物反应器国家重点实验室专项(2060204) （2006AA02Z243）
