江西农业大学学报2011，Vol.33Issue(5)：993-998,6.
经基因工程改造的花生主要过敏原Arah2制备及其低致敏原性鉴定
Preparation and Characterization of the Genetically Engineered Hypoallergenic Derivative of the Major Peanut Allergen Ara h 2
摘要
Abstract
The genetically engineered major allergen Ara h 2 was expressed and purified and the hypoal-lergenic of purified recombinant F - Ara h 2 protein was characterized. The Ara h 2 was reassembled and inserted into the expression vector pET -32a( + ). The vector was transformed into Origami and the protein expression was induced by IPTG. Ni2+ Chelating affinity chromatography was used to purify the recombinant F -Ara h 2 protein. The hypoallergenic of F - Ara h 2 was examined by Western blotting and ELISA. The ORF which contained 471 bp and encoded 157 atnino acids was authenticated to be F - Ara h 2. The recombinant F - Ara h 2 protein induced by IPTG was consistent with the actual value. The affinity between recombinant F -Ara h 2 protein and IgE antibodies from pooled peanut - allergic patients serum was significantly decreased compared with R - Ara h 2 was identified by Western blotting and ELISA. Recombinant F - Ara h 2 protein was hypoallergenic.关键词
基因工程/花生/过敏原/Ara h 2/低致敏Key words
genetic engineering/ peanut/allergen/Ara h2/ hypoallergenic分类
生物科学引用本文复制引用
易海涛,刘芳,贾梦阳,汤慕瑾,曹小勇,夏立新,刘志刚..经基因工程改造的花生主要过敏原Arah2制备及其低致敏原性鉴定[J].江西农业大学学报,2011,33(5):993-998,6.基金项目
国家自然科学基金(30871752)、深圳出入境检验检疫局科技计划项目(SZ2008105)和深圳大学创新团队基金资助项目(200904) （30871752）
