江西农业学报2011，Vol.23Issue(10)：1-5,5.
野蚕孵化酶基因的克隆与生物信息学分析
Cloning and Bioinformatic Analysis of Hatching Enzyme Gene in Chinese Wild Silkworm (Bombyx mandarina)
摘要
Abstract
By means of RT-PCR technology, we cloned a 885 bp (in length) hatching enzyme (HE) gene from the embryo of Chinese wild silkworm (Bombyx mandarina), and this gene was designated as BmandHE (GenBank accession; JN620366). BmandHE ORF encodes 294 amino acids residues with the predicted molecular weight of 33.57 kD and the isoelectric point of 5.55. The deduced BmandHE sequence had a signal peptide of 16 amino acids in N terminator. The BmandHE sequence contained zinc - binding domain and Met - turn domain, which are the characteristic domains of HE. Moreover, it had four cysteine amino acid residues in the fixed positions, which are the backbone for the second structure of HE. Homologous analysis results of the deduced protease domain showed that BmandHE had identity of 30.4% ~97. 1% to the HE in other species. Evolution analysis revealed that Bombyx mandari-. Na had close relation with Bombyx mori, Antheraea pernyi and Culex quinquefasciatus, and they had close genetic relationship.关键词
野蚕/孵化酶基因/ORF/克隆/生物信息学分析Key words
Bombyx mandarina/Hatching enzyme gene/ORF/Cloning/Bioinformatic analysis分类
农业科技引用本文复制引用
唐顺明,赵新慧,吴俊,裘智勇,沈兴家..野蚕孵化酶基因的克隆与生物信息学分析[J].江西农业学报,2011,23(10):1-5,5.基金项目
国家高技术研究发展计划863重点项目(2007AA100504) （2007AA100504）
江苏省自然基金项目(SBK200930217) （SBK200930217）
江苏省高校自然科学基金项目(08KJB230001). （08KJB230001）
