农业环境科学学报2011，Vol.30Issue(10)：2081-2090,10.
转Chi+Glu双价基因棉对土壤微生物群落功能多样性的影响
Effects of Transgenic Chi+Glu Cotton on Functional Diversity of Soil Microbial Community
摘要
Abstract
Through studying effects of transgenic Chi+Glu cotton on functional diversity of soil microbial community, we aimed to reveal the safety of transgenic cottons on the soil ecology environment. The functional diversity of soil microorganism community of transgenic Chi+Glu cotton, transgenic Bt cotton and un-transgenic cotton in different growing stages were analyzed by Biolog Metabolic Fingerprinting method. Although there was no significant difference in the number of soil microorganism community between two transgenic cottons, the number of soil bacteria and actinomycetes of transgenic cotton were significantly higher than that of un-transgenic cotton in stages of flowering and boll-forming stages, while the number of soil fungi was significantly lower than un-transgenic cotton. Compared with un-transgenic cotton, transgenic cotton had less effects on the carbon source utilization ability, Shannon functional diversity index, Simpson index and Mclntosh index of soil microbes at sowing, seeding, squaring and boll-opening stages, but increased the carbon source utilization ability, Shannon functional diversity index and Mclntosh index at flowering and boll-forming stage significantly, while its Simpson index was significantly lower in the flowering and squaring stages. The principal component analysis showed that the 31 carbon sources utilized by the transgenic and un-transgenic cottons had significant difference in the flowering stage. In the squaring stages, the transgenic cotton had a higher utilization of car-boxylic acids, amino acids and amines than that in un-transgenic cotton. It also indicated that in the boll-opening stage, the transgenic cotton had a higher utilization of carbohydrates and carboxylic than un-transgenic cotton and the two transgenic cottons had a low utilization of car-bon sources. The result revealed that transgenic Chi+Glu cotton had significant effects on the soil microorganism community in the flowering and boll-forming stages, while, had no significant difference with transgenic Bt cotton.关键词
棉花/土壤微生物群落/Biolog/主成分分析Key words
cotton/ soil microbial community/ Biolog/ principal component analysis分类
农业科技引用本文复制引用
魏锋,朱荷琴,肖蕊,杨家荣..转Chi+Glu双价基因棉对土壤微生物群落功能多样性的影响[J].农业环境科学学报,2011,30(10):2081-2090,10.基金项目
国家转基因专项“转基因抗病棉花环境安全评价技术研究”(2009ZX08011-026B) （2009ZX08011-026B）
高等学校学科创新引智计划项目(B07049) （B07049）
