中国医学科学院学报2011，Vol.33Issue(5)：538-542,5.DOI:10.3881/j.issn.1000-503X.2011.05.012
成骨细胞在两种胶原支架材料上的生长特征
Growth of Osteoblasts Following Culture on Two Collagen Scaffolds
摘要
Abstract
Objective To explore the growth of osteoblasts following culture on two absorbable collagen scaffolds. Methods MC3T3-E1 osteoblast cell line was inoculated on two collagen scaffolds (BME-10X? And Bio-Gide? ) and co-cultured in vitro. The adhesion and migration of cells were detected by optical microscope. Cell proliferation was detected using CCK-8 reagent kit. The ultrastructure of the adhesion between cells and scaffolds were observed using electronic microscopy. Results MC3T3-E1 osteoblast cell line could adhere , migrate, and proliferate on both two membranes. The proliferation of cells showed no significant difference between porous layer and nonporous layer of BME-1 OX (P) 0.05), while the rate of proliferation was significantly different between loose layer and dense layer of Bio-Gide? ( P < 0. 05 ) . Conclusion Both BME-10X and Bio-Gide have good biocompatibility with MC3T3-E1 osteoblast cell line, and the double-layer structure of Bio-Gide? Can prevent the cells to grow into the dense layer.关键词
成骨细胞/生物相容性/引导组织再生/牙周组织工程Key words
osteoblasts/ biocompatibility/ guided tissue regeneration/ periodontal tissue engineering分类
医药卫生引用本文复制引用
马超,张丁,李平,李小姣..成骨细胞在两种胶原支架材料上的生长特征[J].中国医学科学院学报,2011,33(5):538-542,5.基金项目
国家自然科学基金(31070829) （31070829）
