安徽农业科学2011，Vol.39Issue(22)：13292-13293,13320,3.
改良CTAB法提取薰衣草基因组DNA研究
Extraction of L.pedunculata Genomic DNA Based on the Modified CTAB Method
任艳利 1腊萍 1张相峰 1尚天翠1
作者信息
- 1. 伊犁师范学院化学与生物科学学院,新疆伊宁835000
- 折叠
摘要
Abstract
[Objective] The aim was to find efficiency and rapid L. Peduncuiata genomic DNA extract method. [ Method ] The genomic DNA samples of 3 L. Peduncuiata cultivars were isolated by modified CTAB method. [ Result ] The results showed that the ratio of OD260/OD280 was about 1.85,yield was 454.50-1093.35 μg/ ml,electrophoresis bands was clear and had no significant tailing phenomenon. The fingerprint figure of DNA digested by restriction enzyme Eco RI showed that the extract DNA could be completely digested. Taking the extract DNA as template, the results of RAPD-PCR amplification by one random primer showed that the detected bands were clear. So the extracted DNA had a good quality for deeply molecular biology study. [ Conclusion] The study can lay theoretical basis for deeply carrying out the biological follow - on test of L peduncuiata.关键词
薰衣草/DNA提取/改良CTAB法Key words
L.peduncuiata/ DNA extraction/ Modified CTAB method分类
农业科技引用本文复制引用
任艳利,腊萍,张相峰,尚天翠..改良CTAB法提取薰衣草基因组DNA研究[J].安徽农业科学,2011,39(22):13292-13293,13320,3.
