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粘毛黄芩丛生芽的诱导及快速繁殖

王淑芳 李凤华 窦全丽

安徽农业科学2011,Vol.39Issue(23):14022,14097,2.
安徽农业科学2011,Vol.39Issue(23):14022,14097,2.

粘毛黄芩丛生芽的诱导及快速繁殖

Induction and Rapid Propagation of Scutellauria viscidula Shoots

王淑芳 1李凤华 1窦全丽1

作者信息

  • 1. 遵义师范学院生物系,贵州遵义563002
  • 折叠

摘要

Abstract

[Objective] To provide theoretical basis for the germplasm cryopreservation and factory propagation of ScuteUaria viscidula Bunge. . [Method] With the tender stems of ScuteUaria viscidula Bunge as explants and MS as basic medium for their starting, shoots and rooting culture , the regeneration system for its in vitro culture was constructed. [Result] The optimal induction medium for Scutellellaria viscidula shoots was 2. 0 mg/L 6-BA and 0. 2 mg/L IAA, the average differentiated rate of shoots was 94. 8% with the average reproduction multiples of 11.6; the best rooting culture medium was 1/2MS + IAA 0.5 mg/L, with the highest rooting rate of 97. 8%. [Conclusion] The study established a rapid and high-efficient asexual regeneration system for ScuteUaria viscidula Bunge.

关键词

粘毛黄芩/丛生芽诱导/快速繁殖

Key words

Scutellaria viscidula Bunge/ Shoots induction/ Rapid propagation

分类

农业科技

引用本文复制引用

王淑芳,李凤华,窦全丽..粘毛黄芩丛生芽的诱导及快速繁殖[J].安徽农业科学,2011,39(23):14022,14097,2.

基金项目

遵义市科技局资助项目“根癌农杆菌介导的CrylA抗虫基因转化生姜的研究”([2010]16号). ([2010]16号)

安徽农业科学

0517-6611

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