中国动脉硬化杂志2011，Vol.19Issue(12)：995-1000,6.
番茄红素上调细胞周期素依赖性激酶4和细胞周期素D1的表达促进人外周血内皮祖细胞周期进展
Effects of Lycopene on Cell Cycle and Expression of Cyclin-dependent Kinase 4 and Cyclin D1 in Endothelial Progenitor Cells of Human Peripheral Blood
蒋恒波 1杨红霞 1莫龙2
作者信息
- 1. 湖南永州职业技术学院附属医院心内科,湖南省永州市425006
- 2. 中南大学湘雅医院心内科,湖南省长沙市410078
- 折叠
摘要
Abstract
Aim To investigate the effect of lycopene in endothelial progenitor cells of human peripheral blood and its underlying molecular mechanism.MothedsThe promotion survival effect of lycopene in different concentra-tions on endothelial progenitor cells was measured by thiazolyl blue (MTT) assay. Cell cycle was analyzed by flow cytometry. The expression levels of cyclin-dependent kinase 4 ( CDK4) and cyclin Dl were detected by reverse transcription polymerase chain reaction (RT-PCR) and real-time polymerase chain reaction (real-time PCR) and Western blot. Results The MTT assay showed that lycopen promoted survival effect on endothelial progenitor cells in a time and dose-dependent manner. Flow cytometry analysis revealed that G[ phase of endothelial progenitor cells was decreased after exposed to different concentrations of lycopene. The expression levels of CDK4 and cyclin Dl were increased significantly after treated with lycopene in a dose-dependent manner using RT-PCR, real-time PCR and Western blot. Conclusion The promotion survival effect of lycopene in endothelial progenitor cells is related to G1 phrase reducing through increased expression levels of CDK4 and cyclin Dl.关键词
番茄红素/内皮祖细胞/细胞周期/细胞周期素依赖性激酶4/细胞周期素D1Key words
Lycopene/Endothelial Progenitor Cells/Cell Cycle/Cyclin-dependent Kinase 4/Cyclin Dl分类
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蒋恒波,杨红霞,莫龙..番茄红素上调细胞周期素依赖性激酶4和细胞周期素D1的表达促进人外周血内皮祖细胞周期进展[J].中国动脉硬化杂志,2011,19(12):995-1000,6.
