中国动脉硬化杂志2011，Vol.19Issue(12)：1001-1004,4.
钙调神经磷酸酶对钙化细胞Ⅰ型三磷酸肌醇受体表达的影响
Regulation of Expression of 1,4, 5 Trisphosphate Receptor I by Calcineurin in Cellular Calcification
李芳 1李绍冰 1苑晓烨 1姚丽霞 1田一帆 1冯会英 1李秀芹 1孙鹏1
作者信息
- 1. 河北省人民医院老年病科,河北省石家庄市050051
- 折叠
摘要
Abstract
Aim To explore the modification of inositol 1,4, 5-trisphosphate receptor type I (IP3R I ) expression by calcineurin in calcification of vascular smooth muscle cells. Methods Rat aortic vascular smooth muscle cells after subculture were randomly divided into control group, calcification group and cyclosporine A (CsA) group. Rat aortic vascular smooth muscle cell calcification model was constructed by NaH2PO4. Expression of IP3R I and calcineurin was determined by RT-PCR and Western blot methods. Alkaline phosphatase (ALP) Activity and Ca2+ concentration were determined by colorimetric method. CaN activity was determined by ELISA. ResultsExpressions of IP3R Iand CaNB mRNA and protein increased in calcification group compared with control group ( P < 0. 01) . Expressions of IP3R I and CaNB mRNA and protein decreased in CsA group compared with calcification group (P <0. 01). Ca concentration and CaN and ALP activities of rat aortic smooth muscle cells increased in calcification group compared with control group (P <0. 01) . ALP activity and Ca2+ concentration increased in CsA group compared with calcification group (P < 0. 05 or P < 0. 01). CaN activity decreased in CsA group compared with calcification group ( P < 0. 01). ConclusionsCaN can enhance the expression of IP3R I mRNA and protein in cell calcification.关键词
细胞钙化/钙调神经磷酸酶/Ⅰ型三磷酸肌醇受体/环孢霉素AKey words
Cell Calcification/Calcineurin/1,4,5-Trisphosphate Receptor I/Cyclosporin A分类
医药卫生引用本文复制引用
李芳,李绍冰,苑晓烨,姚丽霞,田一帆,冯会英,李秀芹,孙鹏..钙调神经磷酸酶对钙化细胞Ⅰ型三磷酸肌醇受体表达的影响[J].中国动脉硬化杂志,2011,19(12):1001-1004,4.
