安徽农业大学学报2011，Vol.38Issue(6)：858-862,5.DOI:34-1162/S.20111025.1027.015
油茶SSR-PCR反应体系建立与优化
Establishment and optimization of SSR-PCR reaction system for oil tea(Camellia oleifera Abel.)
摘要
Abstract
Camellia oleifera Abel. Is an edible oil tree species native to China. SSR-PCR reaction system for C. Oleifera was established and optimized through orthogonal test and single factor experiment. The results re vealed that the optimal SSR-PCR reaction system was 10.0 L volume containing DNA (5-10 ng· L-1) 1.0 L, Mg2+ (25 mmol·L-1) 0.7 L, dNTP (10 mmol·L-1) 0.15 L, F- and R-primer (10 mol·L -1) 0.2 L, respectively, Taq DNA (5 U· L-1) 0.1 L, 10 PCR buffer 1.0 L, HPLC H 2O 6.65 L. The cross-species transferability of SSR loci within genus Camellia was validated. Information of the study may offer theoretical base and technical sup port to assessment of genetic diversity, identification of variety, phylogenetic study and QTL analysis of C. Oleif era in the future.关键词
油茶/微卫星标记/PCR反应体系/优化Key words
Camellia oleifera Abel./ SSR/ PCR reaction system/ optimization分类
农业科技引用本文复制引用
刘冰,金龙,曹翠萍,王勤,周明善,吴山忠,束庆龙,张良富..油茶SSR-PCR反应体系建立与优化[J].安徽农业大学学报,2011,38(6):858-862,5.基金项目
安徽省人才开发专项资金(2008Z023),安徽农业大学引进与稳定人才科研资助(yj2008-3),安徽省自然科学基金(090411025)共同资助. （2008Z023）
