安徽农业科学2011，Vol.39Issue(30)：18419-18420,18450,3.
红锥、白椎、大叶栎3个树种分子鉴定及亲缘关系的ISSR分析
Molecular Identification of Castanopsis hystrix, Castanopsis carlesii and Quercus griffithii Using ISSR-PCR Method
摘要
Abstract
[Objective] This research aimed to develop molecular identification method for Castanopsis hystrix, Castanopsis carlesii and Quercus griffithii. [Method] DNA fingerprints of C. Hystrix, C. Carlesii and Q. Griffithii were established using ISSR-PCR method. Cluster Analysis was carried out using UPGMA method based on Nei' s genetic distances among each individual. [Result] Six polymorphic primers were selected from 50 ISSR primers for ISSR-PCR amplification, and totally 86 discernible DNA bands were amplified with 53 polymorphic bands, accounting for 61.2% of the total. The average number of DNA bands amplified by each primer was 10.75. Specifically, totally 5 primers had amplified differential bands and specific bands, which were able to accurately identify C. Hystrix, C. Carlesii and Q. Griffithii. As calculated by DPS v3.01 software , the genetic distances among test materials were ranged from 0. 166 67 to 0.809 52, with an average of 0.563 57. [ Conclusion ] ISSR-PCR method can be used to identify C. Hystrix, C. Carlesii and Q. Griffithii effectively.关键词
壳斗科/红锥/白椎/大叶栎/ISSR/指纹图谱Key words
Fagaceae/ C. Hystrix/ C. Carlesii/ Q. Griffithii/ ISSR/ DNA fingerprint分类
农业科技引用本文复制引用
刘海龙,陈晓明,覃子海,杨开太,林建新,黄金使..红锥、白椎、大叶栎3个树种分子鉴定及亲缘关系的ISSR分析[J].安徽农业科学,2011,39(30):18419-18420,18450,3.基金项目
广西林科院基本业务费项目“红锥群体遗传学研究”(林科200901). （林科200901）
