安徽农业科学2011，Vol.39Issue(28)：17225-17227,3.
墨兰根状茎增殖及生物反应器培养的可行性研究
Rhizome Proliferation of Cymbidium sinense and Feasibility of Bioreactor Culture
摘要
Abstract
[Objective] The aim was to explore the effect of 6-BA concentrations on rhizome proliferation of Cymbidium sinense and feasibility of rhizome culture in bioreactor for providing mass propagules during industrial seedling production. [ Method] The rhizomes of C. Sinense cv. ' Qihua' in vitro were used as experimental material to investigate the effect of 6-BA concentrations on rhizome proliferation and compare different methods of culturing rhizome as well as solid culture, shake culture, and bioreactor culture. [Result] 2.0 mg/L 6-BA was benefit to rhizome proliferation and growth, the impact of proliferation was significant. New rhizomes formed from explants formed after 10 days of culture, and the formed rhizome numbers increased at 10-20 days; at 20 -40 days, the new rhizomes did not differentiate but rhizome elonga tion was found. Rhizome proliferation and growth was promoted by bioreactor culture, 20.6 of rhizomes per explant formed in bioreactor while 13.3 and 5.8 rhizomes per explants were harvested in shake and solid culture, respectively. [Conclusion] 2.0 mg/L 6-BA was favorable for rhizome proliferation in vitro and using bioreactor to mass produce rhizomes is an efficient way for industrial seedling production of C. Sinense.关键词
墨兰/根状茎/生长动态/生物反应器Key words
Cymbidium sinense Willd. / Rhizome/ Dynamic of growth/ Bioreactor分类
农业科技引用本文复制引用
郑艳艳,朴炫春,高日,李美兰,廉美兰..墨兰根状茎增殖及生物反应器培养的可行性研究[J].安徽农业科学,2011,39(28):17225-17227,3.基金项目
国家自然基金项目(30860176). （30860176）
