安徽农业科学2011，Vol.39Issue(23)：14233-14234,14252,3.
海洋硅藻叶绿素a测定方法的改进
Study on the Improvement of Chlorophyll a Measuring Method in the Marine Diatom
施建乐 1朱明新 1蒋皎梅 1徐炎华1
作者信息
- 1. 南京工业大学环境学院,江苏南京210009
- 折叠
摘要
Abstract
[Objective] The research aimed to study the improvement of chlorophyll a measuring method in the marine diatom. [ Method ] The existing chlorophyll a measuring methods which included the criterion method, soaking method and freeze-thaw method were contrasted and analyzed. By combining with the self characteristic of diatom cell, the measuring method of chlorophyll a in the marine diatom was improved. [ Result ] The manual oscillation in the original freeze-thaw method and soaking method was changed into the vortex oscillation 20 min. Moreover, the soaking time in the soaking method decreased to 6 h. The contrast and analysis showed that RSD of improved freeze-thaw method and soaking method was lower than 5% , which had the further decrease than that of original freeze-thaw method and soaking method. Moreover, the relative value of chlorophyll a was higher. [ Conclusion ] The improved freeze-thaw method and soaking method were more suitable for the measurement of chlorophyll a content in the marine diatom. They had the good repeatability. The relative accuracy of measured result was high, and the operation was simple. They were suitable for the experiment whose data was large.关键词
硅藻/叶绿素a/浸泡法/冻融法/改进Key words
Diatom/ Chlorophyll a/ Soaking method/ Freeze-thaw method/ Improvement分类
资源环境引用本文复制引用
施建乐,朱明新,蒋皎梅,徐炎华..海洋硅藻叶绿素a测定方法的改进[J].安徽农业科学,2011,39(23):14233-14234,14252,3.
