安徽农业科学2011，Vol.39Issue(28)：17288-17290,3.
思茅松毛虫2龄幼虫肠道好氧细菌的筛选及毒力测定
Screening and Toxicity Determination of Intestinal Aerobic Bacteria from 2nd instar Larva of Dendrolimu.kikuchii
摘要
Abstract
[Objective ] The paper was to screen intestinal aerobic bacteria from 2 instar larva of Dendrolimu. Kikuchii and determine its toxicity. [Method] The intestinal samples of 2°* instar larva of A kikuchii was diluted in 2-fold and coated on plates to isolate strains, and a total of 5 strains of aerobic bacteria were isolated. With bacterial genomie DNA as template, the universal primers of bacterial 16S rDNA (27f and 1492r) was used to amplify the template ,4 restriction enzymes Hae IH and Hind W ,Hin( I and Taq I were used to carry out ARDRA polymorphism analysis on PCR products. [ Result] Cluster map analysis showed that 5 strains of aerobic bacteria clustered into two different taxonomic operating units (OUT) on 95% similarity level,this indicated that the genetic diversity level of intestinal aerobic bacteria from 2nd instar larva of D. Kikuchii was relatively low. The indoor toxicity test showed that the death peaks of insects killed by intestinal bacteria were during 4-10 d.the insecticide effect of strain 4 was the best with corrected mortality of 53. 57% at 12 d. [ Conclusion] The paper provided reference for control of D. Kikuchii.关键词
思茅松毛虫/肠道细菌/多样性/毒力Key words
Dendrolimu. Kikuchii/Intestinal aerobic bacteria/Diversity/Toxicity分类
农业科技引用本文复制引用
张武先,王金华,熊智,孙佑赫,李彪,赵丽芳,王海林..思茅松毛虫2龄幼虫肠道好氧细菌的筛选及毒力测定[J].安徽农业科学,2011,39(28):17288-17290,3.基金项目
云南省森林灾害预警与控制重点实验室开放基金项目(ZK09A102) （ZK09A102）
国家自然科学基金项目(31100007). （31100007）
