安徽农业科学2011，Vol.39Issue(23)：14256-14259,4.
响应曲面法优化溶藻细菌发酵条件的研究
Optimization of Fermentation Condition of Algicidal Bacteria using Response Surface Methodology
摘要
Abstract
[Objective] To investigate cyanobacteria bloom removal effect of the algicidal bacteria and provide the theoretical and technical basis for the algicidal bacteria,the fermentation condition of the algicidal bacteria R2 were optimized. [ Method] Response surface methodology was employed to optimize the fermentation conditions of algicidal bacteria R2 which separated by the lab. A full factorial design was used to investigate the effect of temperature,initial pH,wave speed,inoculum and medium volume on the growth of algicidal bacteria. [ Result] The temperature and initial pH had significant effect on protease production, both of them had positive effect. The optimized fermentation conditions of the algicidal bacteria R2 were temperature 31.8;pH 7.21;shake speed 180 r/min;mediun volume 60% ;inoculum 10%. The maximum increase of OD600 of algicidal bacteria was 2.310 under the optimized conditions after 24 h. Under the optimum conditions for fermentation,the removal rate of Chl-a was 78%. [ Conclusion ] Using respone surface methodology can effectively optimize the fermmtation conditions of the algicidal bacteria. On the optimized fermentation conditions, the growth condition and the lytic effect of the algicidal bacteria can reach a certain level.关键词
溶藻细菌/响应曲面法/优化Key words
Algicidal bacteria/Response surface methodology/Optimization分类
资源环境引用本文复制引用
叶姜瑜,张将,钟以蓉..响应曲面法优化溶藻细菌发酵条件的研究[J].安徽农业科学,2011,39(23):14256-14259,4.基金项目
三峡工程生态环境建设与保护试点示范专项计划. （）
