安徽农业科学2011，Vol.39Issue(29)：17769-17772,4.
基于ISSR标记的64份广东桑地方品种遗传关系分析
Analysis of Genetic Relationship of 64 Local Varieties of Morus atropurpurea Roxb.Based on ISSR Marker
摘要
Abstract
[Objective] The study aimed at analyzing the genetic relationship of 64 local varieties of Morus atropurpurea Roxb. From the Pearl River Basin in Guangdong and Guangxi Provinces. [ Method] Genetic diversity of 64 local varieties of Morus atropurpurea Roxb. Was analyzed by ISSR molecular marker technique. The genetic relationship among these local varieties was researched by UPGMA method based on the genetic similarity coefficient. [Result] 128 bands were amplified from the total DNA of 64 local varieties using 13 ISSR primers, of which 109 bands accounting for 85. 15% were polymorphic. It meant that there was rich genetic diversity among the local varieties tested. The generic similarity coefficients among 64 local varieties were relatively high with a range of 0.500 0-0. 929 7. In addition, 64 local varieties were divided into two categories and the second could be further divided into 10 subcategories. It was suggested that the genetic relationship of 64 local varieties of Mortis atropurpwea Roxb. Based on ISSR marker analysis had a certain correlation with geographical distribution. [ Conclusion] ISSR marker technology was suitable for evaluating genetic relationship and genetic diversity of local varieties of Morus atropurpurea Roxb. In Pearl River Basin, and could provide scientific basis for DNA fingerprinting and identification of varieties of Morus atropurpurea Roxb.关键词
广东桑/地方品种/ISSR/聚类分析/遗传关系Key words
Morus atropurpurea Roxb. / Local variety/ ISSR/ Cluster analysis/ Genetic relationship分类
农业科技引用本文复制引用
张林,高丽丽,潘一乐,刘利,赵卫国,潘刚..基于ISSR标记的64份广东桑地方品种遗传关系分析[J].安徽农业科学,2011,39(29):17769-17772,4.基金项目
农业部作物种质资源保护项目(NO:NB2010-2130135-24). （NO:NB2010-2130135-24）
