安徽农业科学2011，Vol.39Issue(29)：17788-17789,17818,3.
甘草RAPD-PCR反应体系正交优化研究
Optimization of RAPD-PCR Reaction System for Glycyrrhiza uralensis Based on Orthogonal Design
摘要
Abstract
[Objective] The aim was to obtain the optimum RAPD-PCR reaction system for Glycyrrhiza uralensis. [ Method] Orthogonal design was adopted to screen the suitable concentration of major factors (dNTPs, primer, Taq polymerase, DNA template) in PCR reaction system. [ Result] The optimal protocol was accomplished by orthogonal design in 25 μl reaction volume containing 10 × PCR buffer solution(include MgCl2) 2.5 μl, 10 mmol/L dNTPs 2.5 μl, 50 ng DNA template 2.0 μl, 10 μmol/L primer 2. 0 μl, 5 U/μl Taq polymerase 0.4 μl; the optimum annealing temperature was 34 ℃. [ Conclusion] Orthogonal design was an effective method for the optimization of RAPD-PCR reaction system for C. Uralensis.关键词
甘草/DNA/RAPD-PCR/反应体系/优化/正交试验Key words
Glycyrrhiza uralensis/ DNA/ RAPD-PCR/ Reaction system/ Optimization/ Orthogonal design分类
农业科技引用本文复制引用
张增福,董建力,李明..甘草RAPD-PCR反应体系正交优化研究[J].安徽农业科学,2011,39(29):17788-17789,17818,3.基金项目
国家“十一五”支撑项目(2006BA106A15-11). （2006BA106A15-11）
