高等学校化学学报2011，Vol.32Issue(11)：2504-2508,5.
巴比妥酸衍生物荧光增强法快速测定牛奶中的三聚氰胺
Rapid and Sensitive Fluorescence Enhancement Method by Barbituric Acid Derivatives for the Determination of Melamine in Milk
摘要
Abstract
A rapid and sensitive fluorescence enhancement method was developed for the determination of melamine in milk. In phosphate buffer solution( pH =7.0) , the hydrogen bond between melamine(M) and 5-(9-anthracenylmethylene) barbituric acid(DBA) can be formed and the fluorescence intensity of melamine increased greatly when DBA presented. Under the optimized conditions, the linear dynamic range, the detec tion limit and the relative standard deviation were 12. 5-1250 μg/L, 7. 5 μg/L and 2. 06% , respectively. Combined with the solid phase extraction monolithic column to pretreat the samples, the average value of re covery rates was 96. 62%. The proposed method has been successfully applied for the determination of melamine in milk.关键词
三聚氰胺/巴比妥酸衍生物/荧光增强Key words
Melamine/ Barbituric acid derivative ( DBA) / Fluorescence enhancement分类
化学化工引用本文复制引用
黄晖,向东山,李丽,李海刚,曾国平,何治柯..巴比妥酸衍生物荧光增强法快速测定牛奶中的三聚氰胺[J].高等学校化学学报,2011,32(11):2504-2508,5.基金项目
国家自然科学基金(批准号:21075093)资助. （批准号:21075093）
