高等学校化学学报2011，Vol.32Issue(11)：2533-2538,6.
AuNPs/Sol-gel复合膜法固定乙酰胆碱酯酶生物传感器检测有机磷农药
Determination of Organophosphorus Pesticides Using a Biosensor Based on Acetylcholinesterase Immobilized by Au Nanoparticles/Sol-gel Composite Membrane
摘要
Abstract
Based on the inhibition effects of organophosphorus pesticides on acetylcholinesterase( AChE) , an amperomerometric biosensor was developed by immobilizing AChE on glassy carbon electrode with the composite film of Au nanoparticals and chitosan-silica hybrid sol-gel. Using acetylthiocholine chloride as the substrate , the electrochemical determination method for organophosphorus pesticide was established with monocro-tophos as a representative model for sensitivity tests. The experimental conditions were optimized according to the current response. The response of biosensor fabricated was related linearly to the concentration of monocro-tophos in the range of 0.5-12. 0 μg/mL with a detection limit of 0.02 μg/mL. The developed biosensor exhibited good reproducibility and acceptable stability, providing a simple method for the analysis of organophosphorus pesticide.关键词
乙酰胆碱酯酶/金纳米粒子/溶胶-凝胶/生物传感器/久效磷Key words
Acetylcholinesterase(AChE) / Au nanopaticle/ Sol-gel/ Biosensor/ Monocrotophos分类
化学化工引用本文复制引用
孙春燕,李宏坤,平红,王二雷,张民伟,刘静波..AuNPs/Sol-gel复合膜法固定乙酰胆碱酯酶生物传感器检测有机磷农药[J].高等学校化学学报,2011,32(11):2533-2538,6.基金项目
国家自然科学基金(批准号:20905031)资助 （批准号:20905031）
