高等学校化学学报2011，Vol.32Issue(11)：2548-2553,6.
基于阳离子型共轭聚合物与酶底物探针的磷酸酯酶检测新方法
Novel Method of Phosphatase Detection Based on Cationic Conjugated Polymer and Enzymatic Substrate Probe
摘要
Abstract
A novel method was developed for phosphatase detection based on cationic conjugated polymer (CCP) and enzymatic substrate. The CCP associated with the fluorescein-labeled anionic adenosine triphos-phate(ATP) by electrostatic attractions and fluorescence resonance energy transfer( FRET) from the CCP to the fluorescein-labeled ATP occurred. When phosphatase was added, it catalyzed the hydrolysis of phosphate groups from its substrate, ATP, and the final product adenosine was neutral, which made the CCP detached from adenosine. Then the FRET efficiency decreased. The results show that the degree of FRET efficiency decrease is related to the concentration of phosphatase. This detection method has the advantages including easy operations, real-time response, and high sensitivity. It can also sensitively assay other enzymes that promote the changes of the charge density of substrates; moreover, it provides a promising application in drug screening based on the inhibition of enzymatic activity.关键词
水溶性共轭聚合物/底物/磷酸酯酶/荧光检测/荧光能量共振转移Key words
Water-soluble conjugated polymer/ Substrate/ Phosphatase/ Fluorescence detection/ Fluorescence resonance energy transfer分类
化学化工引用本文复制引用
张小平,黄艳琴,樊春海,黄维..基于阳离子型共轭聚合物与酶底物探针的磷酸酯酶检测新方法[J].高等学校化学学报,2011,32(11):2548-2553,6.基金项目
国家“九七三”计划项目(批准号:2009CB930601)、国家自然科学基金(批准号:20804018,51073078)、江苏省自然科学基金(批准号:BK2008452)、江苏省高等学校优秀科技创新团队项目(批准号:TJ207035)和江苏省高等学校自然科学研究项目(批准号:10KJB150014)资助. （批准号:2009CB930601）
