高等学校化学学报2011，Vol.32Issue(11)：2562-2567,6.
二{[5,5'-10,20-二苯基-15-(4-吡啶基)卟啉]Zn(Ⅱ)]}丁二炔近红外荧光化合物的合成及光学性质
Synthesis and Optical Property of Bis { [ 5,5'-10,20-bis (phenyl) -15 (4-pyridyl) porphyrin ] Zinc ( Ⅱ ) } Butadiyne
摘要
Abstract
Near-infrared fluorescence technology has been applied in biomarkers, bioanalysis, cell imaging and bio-tissue imaging in vivo. Especially the fact that organisms have low scattering effect and background in terference in near-infrared region, increases near-infrared image sensitivity and penetrating depth in biological tissue. Near-infrared fluorescence agents are promising reagent for detection and imaging in deep tissues and organs. Porphyrins have extension electronic conjugated structure, good optical stability, acceptable fluorescent quantum yields, and good biocompatibility, which endow porphyrins with near-infrared emission have more advantages than other dyes in fluorescence imaging. In this paper, bis{ [5 ,5'-10,20-bis(phenyl)-15(4-pyridyl) porphyrin ] zinc (EM butadiyne was designed and synthesized as near-infrared fluorescent compound. The structures of the compounds were confirmed by means of H NMR and MS. UV-Vis spectra were measured by spectral method and fluorescence imaging was recorded by HWHR optical instrument. Deep fluorescence imaging in vitro was studied with biotissue as model. The results show that bisj [5,5'-10,20-bis(phenyl)-15 (4-pyridyl)porphyrin] zinc( Ⅱ ) ! Butadiyne has strong fluorescence in near-infrared region. Fluorescence imaging of biotissue in vitro can achieve structural information in a depth more than 1 cm. So this butadiyne linked prohyrin dimmer partially meets the demand of near-infrared imaging and can be potentially applied in the fluorescence imaging diagnosis in vivo in future.关键词
卟啉/近红外/荧光成像/生物组织Key words
Porphyrin/ Near-infrared/ Fluorescence imaging/ Biotissue分类
化学化工引用本文复制引用
吕丰,陈国辉,刘天军..二{[5,5'-10,20-二苯基-15-(4-吡啶基)卟啉]Zn(Ⅱ)]}丁二炔近红外荧光化合物的合成及光学性质[J].高等学校化学学报,2011,32(11):2562-2567,6.基金项目
国家“九七三”计划项目(批准号:2006CB705703)和教育部博士学科点基金(批准号:20070023020,20101106120052)资助. （批准号:2006CB705703）
