安徽农业科学2011，Vol.39Issue(29)：17944-17948,5.
鱼类致病性迟钝爱德华氏菌中多药抗性质粒pEIB202的消除
Elimination of Multidrug Resistant Plasmid pEIB202 in Fish Pathogenic Edwardsiella tarda
摘要
Abstract
[Objective] The aim of the research was to investigate the function of large plasmid pEIB202 in in the pathogenesis of Edwardsiella tarda EIB202 and eliminate the plasmid pEIB202,so as to lay the foundation for developing safe and live attenuated vaccine against E. Tarda. [Method] sacB was used as reverse screening marker to eliminate the plasmid by using homologous recombination technique. [Result] The plasmid pEIB202 was sequenced and it was found that the plasmid encoded multiple resistant genes and some components in type IV secretion system(T4SS), which suggested that the plasmid might be related with the multiple drug-resistance and pathogenicity of E. Tarda. The plas-mid-eliminated strain ElB202Ap lost the resistance to chloramphenicol and tetracycline, but its growth, virulence and secretion of extracellular proteins had no significant difference with wild-type plants. [Conclusion] pEIB202 plasmid was the main reasons that caused the multi-drug resistance of EIB202 and might have indirect effects in the pathogenesis of EIB202.关键词
迟钝爱德华氏菌(Edwardsiella tarda)/pEIB202/T4SS/R质粒/疫苗Key words
Edwardsiella tarda/ pEIB202/ T4SS/ R plasmid/ Vaccine分类
农业科技引用本文复制引用
郑浚源,许黎黎,王启要,肖婧凡..鱼类致病性迟钝爱德华氏菌中多药抗性质粒pEIB202的消除[J].安徽农业科学,2011,39(29):17944-17948,5.基金项目
国家鲆鲽类产业技术体系(CARS-50) （CARS-50）
国家高技术研究计划(863计划)重点项目:迟钝爱德华氏菌毒力相关基因的鉴定及疫苗开发(2008AA092501). （863计划）
