肿瘤防治研究2011，Vol.38Issue(11)：1283-1287,5.DOI:10.3971/j.issn.1000-8578.2011.11.017
pAkt、Skp2和P27kip1蛋白在肝细胞癌中的表达及意义
Expression and Clinical Significance of pAkt, Skp2 and P27kip1 Protein in Hepatocellular Carcinoma
摘要
Abstract
Objective To explore the expression and clinical significance of pAkt, Skp2 and P27kipl in hep-atocellular carcinoma (HCC) patients. Methods The expression levels of pAkt, Skp2 and P27kipl in 78 HCC and 21 normal liver tissues were evaluated by immunohistochemistry. The relationship between these molecules and clinicopathological variables was further analyzed. Results The positive expression rates of pAkt and Skp2 in HCC were 43. 6% and 47. 4% respectively, and significantly higher than those in normal liver tissue (P<0. 05). P27kipl was expressed in 34. 6% samples of HCC, and significantly lower than that in normal liver tissue (P<0. 05). pAkt expression level was correlated with tumor size, invasion (or lymph node metastasis) and TNM stage. Skp2 expression level was correlated with tumor size, cancer-embolus in portal vein and TNM stage. P27kipl expression level was correlated with tumor size, tumor number and TNM stage. Moreover, There was a positive correlation between Skp2 and pAkt expression level (r = 0. 356, P = 0. 001), while an inverse correlation between Skp2 and P27kip1 expression level (r= - 0. 313, P = 0. 005). HCC patients with high pAkt and Skp2 expression could survive much longer than those with low pAkt and Skp2 expression (P = 0. 000, Log-rank test). No significant difference of survival time was observed within different P27kipl expression groups (P>0. 05, Log-rank test). A multivariate analysis based on Cox regression model demonstrated that TNM stage, pAkt and Skp2 expression might be independent factors affecting the survival time of HCC patients. Conclusion The expression levels of pAkt, Skp2 and P27kipl were closely correlated to the malignant biological behavior of HCC. Skp2 and pAkt were potential biomarkers evaluate the prognosis of HCC.关键词
癌,肝细胞/免疫组织化学/pAkt/S期激酶相关蛋白2/P27kip1Key words
Hepatocellular carcinoma/ Immunohistochemistry/ pAkt/ Skp2/ P27ldpl分类
医药卫生引用本文复制引用
张萌,彭利,乔治斌,何宏涛,周烨,徐卓,杨涛,梁占强..pAkt、Skp2和P27kip1蛋白在肝细胞癌中的表达及意义[J].肿瘤防治研究,2011,38(11):1283-1287,5.基金项目
河北省高校强势特色学科资助项目[2005(52)] （52）
河北省卫生厅资助课题(06142) （06142）
