肿瘤防治研究2011，Vol.38Issue(11)：1245-1248,4.DOI:10.3971/j.issn.1000-8578.2011.11.007
蟾蜍毒素联合紫杉醇抑制人胃癌MGC803细胞增殖和诱导凋亡的作用
Bufalin and Paclitaxel Inhibited Proliferation and Induced Apoptosis of Gastric Cancer Cell Line MGC803
摘要
Abstract
Objective To investigate the effects of bufalin and Paclitaxel on proliferation and apoptosis of gastric cancer cell line MGC803. Methods MGC803 treated by various concentrations of bufalin and Paclitaxel alone or in combination. The inhibition rate of cell growth detected by MTT assay, the apoptosis by flow cytometry and the protein expression of cell apoptosis associated proteins C-FLIPL and Caspase-8 by Western blot Results Bufalin or Paclitaxel effectively inhibited the growth of MGC803 gastric cancer cells in a concentration-dependent manner at certain range of concentrations. The inhibitory rate of bufalin combined with Paclitaxel was significantly higher than that of single drug group(P<0. 05). The values of combination index (CD at 24 h,48 h and 72 h were 0. 77,0. 86 and 0. 72 respectively. The apoptosis rate at 24 h induced by 25 ng/ml Paclitaxel and 40 nmol/L bufalin was 19. 74% and 14. 13% respectively, and lower than that induced by combined treatment (53. 30%,P<0. 05). According to control group as a baseline, C-FLIPL protein expression of Paclitaxel group, bufalin group and combined group were 78. 38%,63. 71% and 46. 73% on the contrary, Caspase-8 protein expression were 150. 25%, 156. 86% and 180. 58% respectively. Conclusion Paclitaxel and bufalin had a syner-gistic effect on growth inhibition and apoptosis gastric cancer MGC803 cells and enhanced Caspase-8 expression and decreased C-FLIP expressioa关键词
胃癌/凋亡/紫杉醇/蟾蜍毒素/C-FLIP/Caspase-8Key words
Gastric cancer/ Apoptosis/ Paclitaxel/Bufalin/ C-FLIP/Caspase-8分类
医药卫生引用本文复制引用
朱志图,王锴,李恩泽,刘阳阳,哈敏文..蟾蜍毒素联合紫杉醇抑制人胃癌MGC803细胞增殖和诱导凋亡的作用[J].肿瘤防治研究,2011,38(11):1245-1248,4.基金项目
辽宁省科技厅2009年博士科研启动资助基金项目(20091051) （20091051）
