肿瘤防治研究2011，Vol.38Issue(11)：1253-1256,4.DOI:10.3971/j.issn.1000-8578.2011.11.009
大蒜素对骨肉瘤MG-63细胞系增殖和凋亡的影响
Effects of Garlicin on Proliferation and Apoptosis of MG-63 Cell Line
摘要
Abstract
Objective To investigate the effects of garlicin on proliferation and apopotosis of human osteo-sarcoma cell line MG-63. Methods Under different garlicin concentrations, the MG-63 cells were observed by inverted microscope) cell counting of kit-8 (CCK-8) assay was used to detect the growth inhibitory rates;and apoptosis was determined by acridine orange/ethidium bromide (AO/EB) double staining and Annexin V-FITC staining flow cytometry (FCM). Cell Cycle was analyzed by FCM. Results Garlicin could significantly inhibit the growth of MG-63 in a dose and time dependent manner and induce G2 / M phase cell cycle arrest. 10 /ng/ml garlicin could induce apoptosis obviously and arrest cell cycle at G2/M phase. Conclusion Garlicin was able to inhibit proliferation and induce apoptosis in MG-63 cells.关键词
大蒜素/细胞凋亡/骨肉瘤/MG-63细胞/细胞周期Key words
Garlicin/ Apoptosis/ Osteosarcoma/ MG-63 cell/ Mitotic cycle分类
医药卫生引用本文复制引用
傅德皓,杨述华,肖宝钧..大蒜素对骨肉瘤MG-63细胞系增殖和凋亡的影响[J].肿瘤防治研究,2011,38(11):1253-1256,4.基金项目
国家自然科学基金资助项目(30901522) （30901522）
