中国畜牧杂志2011，Vol.47Issue(1)：1-5,5.
白羽番鸭Apo VLDL-Ⅱ基因内含子1的克隆及其多态与生长、肉质和脂肪性状的关联分析
Cloning and Polymorphisms of Apo VLDL-H Gene Intron 1 and Its Correlation with Growth,Meat Quality and Fatness Traits in White Muscovy
摘要
Abstract
The full-length of ApoVLDL-II gene intron 1 of White Muscovy was amplified and cloned with the primer A-inl, and its polymorphisms were detected with the primer Al to A7 by PCR-SSCP method, then its correlation with growth, meat quality and fatness traits of White Muscovy were analysed. The results showed that ApoVLDL-II gene intron 1 of White Muscovy was 1362 bp, and its identity with duck and chicken were 93.6% and 69.2%, respectively. The A803G and insertions or deletion AAAATCTTGTTTA after positions of 770 bp in intron 1 were found in the products of primer A4, which resulted in 3 genotypes. The A4 locus was moderate polymorphic and disagreement with Hardy -Weinberg equilibrium (P < 0.05). Association analysis revealed that the CC genotype was significantly lower than DD genotype for pH-value and percentage of chest muscle water (P < 0.05), but intramuscular fat, serum triglyceride and serum cholinesterase was significantly higher than DD genotype (P < 0.05). The CD genotype was significant lower than DD genotype for percentage of chest muscle water (P < 0.05). Furthermore, no significant association of different genotypes with other traits were detected (P > 0.05). So, all of results indicated that ApoVLDL-II gene intron 1 of White Muscovy may play an important role in lipid metabolism, which CC and C may be advantage genotype and allele.关键词
ApoVLDL-Ⅱ/PCR-SSCP/关联分析/白羽番鸭Key words
ApoVLDL-II/PCR-SSCP/association analysis/ White Muscovy分类
农业科技引用本文复制引用
张依裕,徐琪,段修军,张海波,孙国波,赵文明,陈国宏..白羽番鸭Apo VLDL-Ⅱ基因内含子1的克隆及其多态与生长、肉质和脂肪性状的关联分析[J].中国畜牧杂志,2011,47(1):1-5,5.基金项目
国家科技部支撑计划重大专项(2006BDA01A09) （2006BDA01A09）
国家科技部支撑计划重点项目(2006BDA14B06) （2006BDA14B06）
国家水禽产业技术体系项目(农科教发[2008]10号) （农科教发[2008]10号）
