中国畜牧杂志2011，Vol.47Issue(3)：6-10,5.
白羽番鸭脂联素基因SNP与肌内脂肪和血清总胆固醇的关联分析
SNP of Adiponectin Gene and Its Association with Intramuscular Fat and Serum Total Cholesterol in White Muscovy
摘要
Abstract
The whole coding region and intron of adiponectin gene were amplified with 4 pairs of primers designed from Cairina moschata DNA sequence (GQ873067) and its polymorphism was detected by PCR-SSCP method, and then its association with intramuscular fat (IMF) and serum total cholesterol (TC) were analysed. Three SNPs (A167G, T290C and G711A) were found. A167G and G711A, which belonged to silence mutations occurred in exonl and exon2 coding region respectively, and T290C mutation was produced in intron. The three SNP loci presented medium polymorphism and were in accordance with Hardy-Weinberg equilibrium (P > 0.05). Association analysis showed that the birds of genotype AA had significantly higher than genotype BB for IMF and TC in A167G locus (P < 0.05). Then, the birds of genotype CC had significantly higher than genotype TT for IMF in T290C locus (P < 0.05). The research suggested that the A167G and T290C loci had significant genetic effect on fatty deposition and may be used as markers of molecular breeding in White Muscovy.关键词
脂联素基因/SNP/关联分析/白羽番鸭Key words
adiponectin gene/ SNP/ association analysis/ White Muscovy分类
农业科技引用本文复制引用
张依裕,徐琪,段修军,张海波,赵文明,孙国波,陈国宏..白羽番鸭脂联素基因SNP与肌内脂肪和血清总胆固醇的关联分析[J].中国畜牧杂志,2011,47(3):6-10,5.基金项目
国家科技部支撑计划重大专项(2006BDA01A09) （2006BDA01A09）
国家科技部支撑计划重点项目(2006BDA14B06) （2006BDA14B06）
现代农业产业技术体系建设专项资金(nycytx-45-04) （nycytx-45-04）
