癌变·畸变·突变2012，Vol.24Issue(1)：25-29,5.DOI:10.3969/j.issn.1004-616x.2012.01.006
邻苯二甲酸单(2-乙基)己酯对MCF-7细胞DNA的氧化损伤
Effects of mono-2-ethylexyl phthalate on oxidative DNA damage in MCF-7 cells
摘要
Abstract
OBJECTIVE: Oxidative DNA-damaging effects of mono-2-ethylexyl phthalate (MEHP) on MCF-7 cells were investigated. METHODS: MCF-7 cells were treated with MEHP at various concentrations (6.25, 12.5, 25, 50 and 100 μ mol/L) or dimethyl sulphoxide alone (DMSO, solvent control, final concentration <1‰). Cell proliferation was measured by the MTT assay at 12 and 24 h after the treatment. At the time points (1.5, 3, 6, 12 and 24 h), the content of malondialdehyde (MDA) and activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were measured using the corresponding kits. The level of (8-hydroxy-2'-deoxyguanosine, 8-OHdG) in MEHP-treated cells was estimated using high-pressure liquid chromatography with electrochemical detection HPLC-ECD method. RESULTS: At 12 h after treatment, cell proliferation rates significantly increased in MEHP-treated groups (6.25 and 12.5 μmol/L, P<0.05 for both). However, cell proliferation rates significantly decreased at 24 h after treatment, in the higher MEHP-treated groups (25-100 μ mol/L,P<0.05 for all). There were no significant changes in the levels of MDA, GSH-Px and 8-OHdG (P<0.05 for all).After MCF-7 cells were treated with MEHP for short periods (1.5, 3 and 6 h), the levels of MDA and 8-OHdG in all treatment groups were increased (P<0.05), but SOD and GSH-Px activities were reduced (P<0.05 for all). CONCLUSION: Short periods(≤ 6 h) of treatment with MEHP at a certain concentration induced oxidative stress in MCF-7 cells, and accelerated DNA oxidative damage. The underlying mechanisms of MEHP mediated oxidative DNA damage MCF-7 cells need to be investigated.关键词
邻苯二甲酸单(2-乙基)己酯/氧化应激/DNA氧化损伤/8-羟基脱氧鸟苷Key words
MEHP/ oxidative stress/ DNA oxidative damage/ 8-OHdG分类
医药卫生引用本文复制引用
郑红燕,饶凯敏,姜英,杨光涛,陈曦,王建书,汪倩,王晶,袁晶..邻苯二甲酸单(2-乙基)己酯对MCF-7细胞DNA的氧化损伤[J].癌变·畸变·突变,2012,24(1):25-29,5.基金项目
国家自然科学基金项目(30972437) （30972437）
