安徽工程大学学报2011，Vol.26Issue(4)：13-16,4.
复合诱变原生质体选育高抑菌活性纳豆菌
Breeding Bacillus natto with high antibacterial activity by compound mutation protoplast
许鹏 1张庆庆 1汤文晶 1吕闻闻1
作者信息
- 1. 安徽工程大学微生物发酵安徽省工程技术研究中心,安徽芜湖241000
- 折叠
摘要
Abstract
To obtain excellent strain with a high antibacterial activity,the composite mutation of Bacillus natto protoplast was studied by UV ray and 5-fluorouracil.The results showed that under the condition of 0.3 mg/ml enzymolysis concentration,40 min enzymolysis time,35 ℃ enzymolysis temperature and pH 7.0 enzymolysis,the pretoplast formation and regeneration rates reached its highest.By compound UV and chemical mutation,a with high antibacterial activity strain was obtained.Compared with the original strain,its antibacterial effect of Staphylococcus aureus increased by 19.7%,and its antibacterial effect of E.coli increased by 19.5%.The antibacterial activity stays stable after 10 generations of reproduction.关键词
原生质体/复合诱变/抑菌活性/纳豆菌Key words
protoplast composite mutation antibacterial activity Bacillus natto分类
生物科学引用本文复制引用
许鹏,张庆庆,汤文晶,吕闻闻..复合诱变原生质体选育高抑菌活性纳豆菌[J].安徽工程大学学报,2011,26(4):13-16,4.
