安徽农业大学学报2012，Vol.39Issue(1)：14-18,5.DOI:34-1162/S.20111226.1620.015
RIP140基因多态性及其与济宁青山羊产羔数关系的研究
Polymorphism of RIP140 gene and its relationship with litter size of Jining Grey goat
摘要
Abstract
According to the Bos taurus RIP 140 mRNA sequence, five pairs of primers were designed to am plify whole CDS sequence of RIP 140 gene in five randomly selected individuals from Jining Grey goats and Liaoning Cashmere goats, respectively. Through DNA sequencing and alignment, the whole CDS sequence of the goat RIP140 gene was obtained. Meanwhile, one nucleotide transition (C822T) was identified in goat RIP140 gene. According to the CDS sequence of the goat RIP 140 gene, primer P6 was designed to detect C822T mutation of RIP 140 gene in high prolificacy breeds (Jining Grey and Guizhou White goats) and low prolificacy breeds (Liaoning Cashmere, Boer and Inner Mongolia Cashmere goats) by PCR-RFLP. The results showed that three genotypes (CC, CT and TT) were detected in Jining Grey, Boer, Liaoning Cashmere and Guizhou White goats and only two genotypes (CT and TT) were detected in Inner Mongolia Cashmere goat. In Jining Grey goat, genotype frequency of CC, CT and TT was 0.056, 0.309 and 0.635, respectively. Jining Grey goat does with genotype CC or CT had 0.81(P<0.05) or 0.65 (P<0.05) kids more than those with genotype TT, respectively. The Jining Grey goat does with genotype CC had 0.16 (P>0.05) kids more than those with genotype CT. These results preliminarily indicated that allele C of RIP140 gene is a potential DNA marker for improving litter size in goats.关键词
山羊/RIP140/高繁殖力/PCR-RFLPKey words
goat/ RIP140 gene/ prolificacy/ PCR-RFLP分类
农业科技引用本文复制引用
唐倩倩,冯涛,储明星,狄冉,方丽..RIP140基因多态性及其与济宁青山羊产羔数关系的研究[J].安徽农业大学学报,2012,39(1):14-18,5.基金项目
国家高技术研究发展计划(863)(No.2006AA 10Z139),国家现代农业产业技术体系建设专项资金(CARS-39),国家重点基础研究发展计划(973)(No.2006CB102105),国家自然科学基金项目(No.30871773),中国农业科学院北京畜牧兽医研究所基本科研业务费专项(2010jc-9)和中国农业科学院首批优秀科技创新团队专项共同资助. （863）
