安徽农业大学学报2012，Vol.39Issue(1)：36-40,5.DOI:34-1162/S.20111226.1621.022
大鲵2个驯养群体ITS2遗传多样性分析
Analysis of ITS2 genetic diversity between two acclimation populations of Chinese giant salamander, Andrias davidianus
摘要
Abstract
The nuclear DNA of two populations of Andrias davidianus was amplified by PCR. The amplified fragments including 5.8S and 28S nuclear ribosomal RNA gene partial sequence and the nuclear ribosomal second internal transcribed spacer (ITS2) complete sequence. The length of the fragment contained 606 bp nucleotides. The genetic diversities of Andrias davidianus from the Changshou Lake Rare Fish Breeding Center (CSLRFBC) of the Chongqing Fisheries Research Institute and Guanghan Rare Aquaculture Company (GHRAC) were ana lyzed. The result indicated as follows. The T, C, A and G average contents were 15.6%, 36.5%, 12.5% and 35.4% respectively. The average contents of G+C and A+T were 71.9% and 28.1% respectively. The average values of transversional pairs were 271 and transitional pairs were 112. The ratio of transition and transversion was 0.41, and the ratio of transversion exceeded that of transition significantly. The 10 individuals belonged to 10 haplo types according to the determined sequences. The haplotypic diversity (H) was 1.000 0±0.1260, and the average number of nucleotide differences (K) of them was 5.932 1 ±0.861 4. The nucleotide diversity (Pi) of the 10 indi viduals was 0.631 9±0.013 9. The correlation within populations relative to total was 0.0305 to the two popula tions. Two populations have not differentiated to a single population by test of Tajima's D, Fu&Li's D, F and clus ter analysis.关键词
Andrias davidianus/大鲵/ITS2/遗传多样性/资源保护Key words
Andrias davidianus/ Chinese giant salamander/ ITS2/ genetic diversity/ conservation分类
农业科技引用本文复制引用
马跃岗,朱杰,袁万安..大鲵2个驯养群体ITS2遗传多样性分析[J].安徽农业大学学报,2012,39(1):36-40,5.基金项目
2010重庆市科委基本科研业务费支助项目(CH001)和重庆市水产研究所支助项目共同资助. （CH001）
