安徽农业大学学报2012，Vol.39Issue(1)：55-60,6.DOI:34-1162/S.20111025.1031.032
狸猫α-干扰素全基因的克隆及生物信息学分析
Cloning and bioinformatics analysis of IFN- α gene of wild cat
摘要
Abstract
One pair of primer was designed and synthesized according to the feline interferon alpha gene (IFN-a) nucleic acid sequence. Wild cat IFN-a was amplified by PCR, then cloned and sequenced. The nucleotide sequence of the amplified IFN-a gene and the amino acid sequence deduced from the gene were then analyzed by the DNAstar software and on-line tools, and the similarity and evolution of IFN-a gene were analyzed among wild cat, human and other animals. The results showed the full length sequence of wild cat IFN-a gene was 631 bp, which includes one open-reading frame, encoding 194 amino acid residues, one potential N-glycosalation site and seven 0-glcnac sites in deduced amino acid sequence. The results of comparative sequence analysis and phyloge netic tree analysis indicated there was difference of genus in IFN-a gene, the nearer their relationship, the higher the homology. This study paved the way for future study of biological function and application of feline IFN-a.关键词
狸猫/α-干扰素基因/基因克隆/生物信息学Key words
wild cat/ interferon-a/ gene cloning/ bioinformatics分类
生物科学引用本文复制引用
朱前磊,王亚丹,崔保安,陈红英,魏战勇,王子馨,段廷云..狸猫α-干扰素全基因的克隆及生物信息学分析[J].安徽农业大学学报,2012,39(1):55-60,6.基金项目
国家"十五"食品安全重大攻关专项(2001BA804A30-11)资助. （2001BA804A30-11）
