安徽农业大学学报2012，Vol.39Issue(1)：79-83,5.DOI:34-1162/S.20111226.1620.020
天目铁木雄花序内源多胺的HPLC分析及其动态变化
Dynamic changes of endogenous polyamines in male anthotaxy of Ostrya rehderiana by HPLC
摘要
Abstract
The contents of endogenous polyamines (putrescine, spermidine and spermine ) in male inflores cence of Ostrya rehderiana were analyzed with HPLC (high performance liquid chromatography) during the dif ferentiation. The results showed that 37℃ and 30 minutes were the best conditions for benzoylation. The chro matographic conditions were as follows: detection wavelength, 254 nm; mobile phase, a mixture of methanol and water (methanol : water = 60:40 ) and acetic acid; velocity of flow, 0.6 mL·min-1; temperature of column, 30℃; injection volume, 5 mL. The detection results showed that the polyamines could be isolated to determine in the conditions. In addition, it was found that during the differentiation the content of spermidine was the most and the putrescine was the least, while three polyamines all increased then decreased obviously. It was effective to detect the content and reveal the trends of three endogenous polyamines of Ostrya rehderiana with optimization HPLC.关键词
天目铁木/雄花序/高效液相色谱法/内源多胺Key words
Ostrya rehderiana/ male inflorescence/ HPLC/ endogenous polyamines分类
农业科技引用本文复制引用
王祖良,陆海根,黄珊珊,黄华宏,楼雄珍..天目铁木雄花序内源多胺的HPLC分析及其动态变化[J].安徽农业大学学报,2012,39(1):79-83,5.基金项目
浙江珍稀濒危树种现代保育关键技术研究(2006C22064)资助. （2006C22064）
