安徽农业大学学报2012，Vol.39Issue(1)：111-115,5.DOI:34-1162/S.20111226.1619.014
不同方法提取吴茱萸叶片基因组DNA和RNA的比较
Comparation on different methods on genomic DNA and RNA extraction from young leaves of Evodia rutaecarpa
摘要
Abstract
In order to definite suitable extraction methods of genomic DNA and RNA from Evodia rutae carpa' s young leaves, three difference methods were compared each other, respectively. Ultraviolet spectropho tometry and ordinary electrophoresis methods were used to detect and compare the extraction effect of genomic DNA on traditional CTAB method, SDS method and modified CTAB method. Ultraviolet spectrophotometry and native gel electrophoresis were used to detect and compare the extraction effect of RNA on phenol-SDS method, Trizol method and modified guanidine thiocyanate method. The result showed that the qualities of genomic DNA and RNA extracted from Evodia rutaecarpd s young leaves were clearly different using different extraction methods. The quality of genomic DNA exacted from Evodia rutaecarpa' s young leaves with CTAB method and SDS method were not satisfied, but the extraction effect was very well using modified CTAB method. The quali ties of RNA extracted from Evodia rutaecarpa's young leaves with Phenoi-SDS method and Trizol method were not high, but the extraction effect was very well using modified guanidine thiocyanate method.关键词
吴茱萸/基因组DNA/RNA/提取方法Key words
Evodia rutaecarpa/ genomic DNA/ RNA/ extraction method分类
医药卫生引用本文复制引用
吴波,高丹,潘超美,张寿文..不同方法提取吴茱萸叶片基因组DNA和RNA的比较[J].安徽农业大学学报,2012,39(1):111-115,5.基金项目
国家"十二五"科技支撑计划项目(2011BAI04B04)资助. （2011BAI04B04）
