安徽农业科学2011，Vol.39Issue(33)：20306-20309,4.
Na2CO3胁迫对甜菊不同碱耐性品种生理代谢的影响
Influences of Na2 CO3 Stress on the Physiological Metabolisms of Different Stevia rebaudiana Alkali Tolerance Varieties
摘要
Abstract
[Objective] The research aimed to discuss the physiological mechanisms of alkali tolerances of different Stevia rebaudiana varieties under the alkali stress. [Method] By using the matrix culture method, the influences of Na2CO3 on leaf chlorophyll content, malondialdehyde (MDA), superoxide dismutase (SOD), peroxidase (POD) and Pro content of different S. Rebaudiana alkali tolerance varieties [Shoutian No. 2 (relative alkali tolerance variety) and Zhongshan No. 4 (alkali-sensitive variety) ] were studied. [ Result ] The alkali stress of 1.2 g/L of Na2CO3 made that the chlorophyll contents of Shoutian No. 2 and Zhongshan No. 4 seedlings both decreased to some extent. Moreover, MDA content of Zhongshan No. 4 was higher than the control during the whole stress period, and the largest increase range was 43. 2%. MDA content of Shoutian No. 2 was lower than the control in early and end periods of stress, and increased the maximum on the 14' day of alkali stress, which was 24.4% higher than the control. SOD activities of Shoutian No. 2 and Zhongshan No. 4 both showed a trend of first increasing and declining then in the alkali stress period, but the increasing extent of SOD activity in Shoutian No. 2 was higher than that in Zhongshan No. 4. In late stage of Na2CO3 stress, SOD activity of Shoutian No. 2 declined, but POD activity was higher than that of Zhongshan No. 4. It illustrated that POD had the strong scavenging capacity of reactive oxygen. Pro contents of Shoutian No. 2 and Zhongshan No. 4 were higher than the control in the stress period. It showed that the osmotic adjustment of Pro may not be key regulatory factor of alkali tolerance difference for the two kinds of S. Rebaudiana. [Conclusion] The research not only provided the theoretical basis for further breeding the new alkali tolerance variety of S. Rebaudiana, but also had the important significance for improving the utilized ratio of kaline soil and environment for beach site in China.关键词
甜菊/Na2CO3胁迫/生理代谢Key words
Stevia rebaudiana/Na2CO3 stress/Physiological metabolism分类
农业科技引用本文复制引用
绳仁立,原海燕,黄苏珍..Na2CO3胁迫对甜菊不同碱耐性品种生理代谢的影响[J].安徽农业科学,2011,39(33):20306-20309,4.基金项目
江苏省科技厅科技支撑项目(BE2009322) （BE2009322）
南京市科技局成果推广项目(200901001) （200901001）
江苏省农业三新工程项目(sx[2011]247). （sx[2011]247）
