安徽农业科学2011，Vol.39Issue(33)：20517-20519,3.
湖北白猪IGF-I基因的克隆及序列分析
Cloning and Sequence Analysis on IGF-I Gene of Hubei White Swine
摘要
Abstract
[Objective] The study aimed at cloning and analyzing the insulin-like growth factor-I (IGF-I) gene from liver of Hubei white swine. [ Method ] The total RNA was extracted by using Trizol from the liver of Hubei white swine and used as template to amplify IGF-I gene Cdna by RT-PCR. The Cdna product was cloned into Pcr(R)Ⅱ vector, screened with blue-white colonies, digested with double enzymes and sequenced. [Result] The sequencing result indicated that the IGF-Ⅰ gene consisted of 607 nucleotides, containing 5'-untranslated region at nucleotides 1-145, a complete ORF at nucleotides 146-538 encoding 130 amino acids, and 3'-untranslated region at nucleotides 539 - 607. It shared 100% homology with the porcine IGF-Ⅰ gene reported by Muller et al. [ Conclusion] The successful cloning and sequencing of the Hubei white swine IGF-Ⅰ gene confirmed that IGF-Ⅰ gene was highly conserved, which provided a technical basis for the use of transgenic technology for breeding of Hubei white swine.关键词
湖北白猪/IGF-I基因/序列分析Key words
Hubei white swine/IGF-Ⅰ gene/Sequence analysis分类
农业科技引用本文复制引用
乔宪凤,张立苹,毕延震,刘西梅,华文君,华再东,肖红卫,周荆荣,郑新民..湖北白猪IGF-I基因的克隆及序列分析[J].安徽农业科学,2011,39(33):20517-20519,3.基金项目
转基因专项(2008ZX08006-002,2008ZX08006-003,2008ZX08010-003,2008ZX08011-004) （2008ZX08006-002,2008ZX08006-003,2008ZX08010-003,2008ZX08011-004）
湖北省重点实验室项目(2011ZD127). （2011ZD127）
