安徽农业科学2011，Vol.39Issue(34)：20951-20952,2.
rhtB基因过表达苏氨酸工程菌发酵过程的优化控制
Optimization of Culture Conditions of rhtB Gene Overexpressed Threonine-producing Strain
摘要
Abstract
[Objective] The aim was to construct key gene rhtB overexpressed threonine-producing E. Coli strains which transported threonine to extracellular translocate, and to investigate the effect of different carbon and nitrogen sources and pH value on L-threonine. [ Method] The fermentation process of different carbon and nitrogen sources on L-threonine yield strains were analyzed to optimize carbon and nitrogen sources of fermentation medium and pH. [Result] Data showed that the suitable carbon source for threonine production by E. Coli M122 was sucrose whose yield of threonine was 28.1 g/L. When ammonium sulfate or yeast extract were acted as nitrogen sources, production reached 27. 8 and 28.2 g/L, respectively. The yield of threonine was pH dependent and occurred at approximately pH 7.0. [Conclusion] The best carbon source for threonine production by E. Coli M122 was sucrose. Either of sources including ammonium sulfate or yeast extract could be used as optimal nitrogen. pH is a critical factor in threonine production, which should be set at 7.0.关键词
L-苏氨酸/发酵/碳源/氮源Key words
L-threonine/ Fermentation/ Carbon source /Nitrogen source分类
农业科技引用本文复制引用
孔晶,贾建波,赵玉萍,任世英..rhtB基因过表达苏氨酸工程菌发酵过程的优化控制[J].安徽农业科学,2011,39(34):20951-20952,2.基金项目
淮安市科技支撑计划(农业)项目(SN1049). （农业）
