安徽农业科学2011，Vol.39Issue(34)：20961-20962,21020,3.
应用正交试验优选茎瘤芥子叶组培诱导培养基
The Optimization of the Optimum Tissue Culture Medium in Stem Lump Mustard Cotyledon by Using Orthogonal Design
摘要
Abstract
[Objective] The study aimed to establish regeneration plant system of stem lump mustard somatic cell culture, to lay the foundation for genetic transformation 9ystem and biotechnology breeding of Brassicas stem lump mustard and leaf mustard. [ Method] Talcing 6 d cotyledon of stem lump mustard as materials, using orthogonal design methods, the effect of different hormone of 6-BA, NAA, 2,4-D on explants differentiation and seedling rate of callus were studied. [ Result ] The best culture medium for the growth of stem lump mustard cotyledon was MS with 6-BA 3.0 mg/L + NAA 0. 10 mg/L + 2,4-D 0 mg/L + 3 g/L agar powder; moreover, 1/2MS supplied with 0. 10 mg/L NAA was good for the rooting of tissue culture seedling. [Conclusion] The regeneration frequency of optimized medium was improved greatly.关键词
正交法:茎瘤芥/组织培养Key words
Orthogonal dcsign/Stem lump mustard:Tissue culture分类
农业科技引用本文复制引用
孙全,朱小燕,梁婷,刘毅,岳显可,何晓红,蔡应繁..应用正交试验优选茎瘤芥子叶组培诱导培养基[J].安徽农业科学,2011,39(34):20961-20962,21020,3.基金项目
国家自然科学基金(31071461,30771311) （31071461,30771311）
重庆市重点攻关项目(CSTC-2011AB1095) （CSTC-2011AB1095）
重庆市自然科学基金(CSTC-2009BA1088,cstc2007BB1328). （CSTC-2009BA1088,cstc2007BB1328）
