安徽农业科学2011,Vol.39Issue(34):20963,20967,2.
野生木薯组织培养快繁技术研究
Study on Tissue Culture and Rapid Propagation Technique for Wild Cassava
摘要
Abstract
[Objective] The study aimed to conduct the germplasm resource conservation and large-scale seedling propagation on the wild cassava. [ Method] The tissue culture tnelhod was use lo make for the rapid propagation. [ Result] The medium suitable for the callus induction and the adventitious bud differentiation of the wild cassava was MS +6-BA 0. 10-0.50 mg/L + NAA 0. 1 -0.5 mg/L; the medium suitable for the proliferation was MS (Fe content doubling) +6-BA0. 50 -0.65 mg/L + IBA0. 1 mg/L + PVP 200 mg/L; the medium suitable for rooting was MS (Fe content doubling) +0. 1% AC and the rooting rate was more than 90% ; when the test tube seedlings was made for the domestication in the matrix with the ratio of the crud river sand to the fine river sand of 2:1, the survival rate was 85%. [ Conclusion] The rapid propagation method for the wild cassava was established.关键词
野生木薯/组织培养/培养基Key words
Wild cassava/ Tissue culture/ Medium分类
农业科技引用本文复制引用
李军,俞奔驰,盘欢,黎萍,吕平..野生木薯组织培养快繁技术研究[J].安徽农业科学,2011,39(34):20963,20967,2.基金项目
国家自然科学基金项目(30860133) (30860133)
广西科学基金项目(桂科自0991223). (桂科自0991223)