安徽农业科学2011，Vol.39Issue(34)：20969-20970,20981,3.
破壁方法对短乳杆菌谷氨酸脱羧酶提取效果的影响
Influences of Cell-wall Breaking Methods on Extraction Effect of Glutamate Decarboxylase from Lactobacillus brevis
摘要
Abstract
Different cell-wall breaking methods were taken for extracting glutamate decarboxylase ( GAD) from Lactobacillus brevis. The effects of cell wall breaking methods on activity of GAD were studied. The results showed that the ultrasonic method was beneficial to the release of GAD from the cells. The results of orthogonal test showed that the best process for cell-wall breaking with ultrasonic was: ultrasonic power of 220 W, working time of 20 min, the ratio of work time/intermission time of 5 s/10 s.关键词
破壁方法/短乳杆菌/谷氨酸脱羧酶Key words
Cell-wall broken method/Lactobacillus brevis/ Glutamate decarboxylase分类
农业科技引用本文复制引用
张颖,高年发,段强,于韶梅..破壁方法对短乳杆菌谷氨酸脱羧酶提取效果的影响[J].安徽农业科学,2011,39(34):20969-20970,20981,3.基金项目
天津市高等学校科技发展基金计划项目(No.20090601). （No.20090601）
